In vitro activities of colistin combined with imipenem, tigecycline or cefoperazone-sulbactam against multidrug-resistant Acinetobacter baumannii blood-stream isolates

  • Oznur Akyol Erikci Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey
  • Demet Hacıseyitoglu Department of Microbiology and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey
  • Yasemin Cag Department of Infectious Diseases and Clinical Microbiology, Medeniyet University Goztepe Training ad Research Hospital, Istanbul, Turkey
  • Serap Gencer Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey
  • Funda Biteker Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey
  • Serdar Ozer Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey
Keywords: Acinetobacter baumannii, colistin, synergy

Abstract

Acinetobacter baumannii has emerged as one of the most important nosocomial pathogens and multi-drug resistant (MDR) isolates are of great concern worldwide. The aim of the present study was to investigate the in vitro synergistic activity of colistin in combination with other antibiotics against MDR A. baumannii blood stream isolates. A total of 54 non-duplicate, MDR A. baumannii isolates from blood culture specimens obtained between June 2011 and July 2012 were included in the study. In vitro synergistic activity of colistin in combination with imipenem, tigecycline or cefoperazone-sulbactam against study isolates was investigated by Etest superimposing method and the fractional inhibitory concentration (FIC) index was calculated for each antibiotic combination. The most frequent synergistic effect of colistin was found in combination with tigecycline in only 7 isolates (13.0%). All three antibiotics were found to have synergistic effect with colistin in four isolates (7.4%). Of isolates, 46 (85.2%) showed additive effect of colistin in combination with cefoperazone-sulbactam or tigecycline, 45 (83.3%) with imipenem. We found synergistic activity of colistin with other antibiotics in only a small number of isolates. Although Etest method is a practical method to investigate the synergistic activity, in case of choosing empirical treatment, colistin in combination with another antibiotic may be preferred.

Author Biographies

Oznur Akyol Erikci, Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Demet Hacıseyitoglu, Department of Microbiology and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Department of Microbiology and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Yasemin Cag, Department of Infectious Diseases and Clinical Microbiology, Medeniyet University Goztepe Training ad Research Hospital, Istanbul, Turkey

Department of Infectious Diseases and Clinical Microbiology, Medeniyet University Goztepe Training ad Research Hospital, Istanbul, Turkey

Serap Gencer, Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Funda Biteker, Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Serdar Ozer, Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Department of Infectious Diseases and Clinical Microbiology, Dr.Lütfi Kirdar Kartal Training and Research Hospital, Istanbul, Turkey

Susceptibility
Published
2016-12-31
How to Cite
Akyol Erikci, O., Hacıseyitoglu, D., Cag, Y., Gencer, S., Biteker, F., & Ozer, S. (2016). In vitro activities of colistin combined with imipenem, tigecycline or cefoperazone-sulbactam against multidrug-resistant Acinetobacter baumannii blood-stream isolates. Disease and Molecular Medicine, 4(4), 51-54. https://doi.org/10.5455/dmm.20160830024755
Section
Original Article