Prevalence of CTX-M enzyme and qnrA, qnrB, qnrC, qnrS, aac-(6)-Ib genes among extended spectrum beta lactamase-positive isolates in patients undergoing transrectal needle prostate biopsy in Turkey

  • Elif Tükenmez Tigen Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey
  • Zafer Tandoğdu Department of Urology, Taksim Training and Research Hospital, Istanbul, Turkey
  • Gulsen Altınkanat Department of Microbiology, Marmara University, Istanbul Turkey
  • Arzu Doğru Department of Infectious Disease and Clinical Microbiology, Goztepe Training Hospital, Istanbul Medeniyet University, Istanbul, Turkey
  • Buket Ertürk Şengel Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey
  • Volkan Korten Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey
Keywords: Beta-lactamase CTX-M, enterobacteriaceae, qnr protein, prostate

Abstract

Fecal carriage is one of the most important reasons for extended spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) causing infections. We aimed to demonstrate epidemiological features for a subtype of ESBL-PE-encoding TEM, SHV, and CTX-M as well as for qnrA, qnrB, qnrC, qnrS, aac-(6)-Ib genes through polymerase chain reaction (PCR) in patients undergoing transrectal needle prostate biopsy (TRNBP). Between October 2008 and February 2010, we collected 400 fecal swabs from patients prior to TRNBP in four separate centers. After detecting ESBL-PE isolates in the material, we further analyzed TEM, SHV, and CTX-M enzymes, as well as three types of qnr genes of qnrA, qnrB, qnrC and aac-(6)-Ib by PCR. We detected 80 ESBL-PE isolates in 400 fecal samples. Of the 80 isolates; blaSHV, blaTEM, and blaCTX-M were observed in 12, 46 and 79 isolates, respectively. All three genes were present in eight isolates. Resistance to quinolone was identified in 67 (83.7%) isolates, resistance to aminoglycoside in 52 (65%) isolates, and resistance to both antibiotics in 46 (57.5%) isolates. Subsequently, we determined qnrB, qnrS and aac-(6)-Ib genes in 7 (8.8%), 11 (14%) and 60 (76%) isolates, respectively. qnrA and qnrC were not detected in any of the isolates. CTX-M-producing ESBL-PE is the most common pathogen responsible for fecal carriage in the community. Plasmid-mediated quinolone resistance genes (qnr, aac-(6)-Ib) are the reason behind the dissemination of ESBLs.

Author Biographies

Elif Tükenmez Tigen, Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

Zafer Tandoğdu, Department of Urology, Taksim Training and Research Hospital, Istanbul, Turkey

Department of Urology, Taksim Training and Research Hospital, Istanbul, Turkey

Gulsen Altınkanat, Department of Microbiology, Marmara University, Istanbul Turkey

Department of Microbiology, Marmara University, Istanbul Turkey

Arzu Doğru, Department of Infectious Disease and Clinical Microbiology, Goztepe Training Hospital, Istanbul Medeniyet University, Istanbul, Turkey

Department of Infectious Disease and Clinical Microbiology, Goztepe Training Hospital, Istanbul Medeniyet University, Istanbul, Turkey

Buket Ertürk Şengel, Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

Volkan Korten, Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

Department of Infectious Disease and Clinical Microbiology, Marmara University, Istanbul, Turkey

beta lactamase producing Enterobacteriaceae
Published
2016-03-31
How to Cite
Tükenmez Tigen, E., Tandoğdu, Z., Altınkanat, G., Doğru, A., Ertürk Şengel, B., & Korten, V. (2016). Prevalence of CTX-M enzyme and qnrA, qnrB, qnrC, qnrS, aac-(6)-Ib genes among extended spectrum beta lactamase-positive isolates in patients undergoing transrectal needle prostate biopsy in Turkey. Disease and Molecular Medicine, 4(1), 1-5. https://doi.org/10.5455/dmm.20160501015830
Section
Original Article