Lack of Association between Glutathione S-Transferase -M1 and -T1 Gene Polymorphisms with Clinicopathological Parameters in Prostate Cancer
Glutathione S-transferase (GST) enzymes play a role in detoxification of several carcinogens. Inherited absence of both alleles are common in human in two subtypes of GST gene superfamily, -M1 and -T1, by varying frequencies among different populations. The absence of both alleles result in loss of enzymatic activity and are related to increased risk for development of various cancer types. In this study, we investigated the association of GSTM1 and GSTT1 genetic profiles with clinicopathological parameters in prostate cancer. GSTM1 and GSTT1 genotypes were assessed by multiplex PCR and high
resolution melting curve analysis method in 162 patients that underwent radical prostatectomy for localised prostate cancer. Their association with prognostic parameters were analysed. Frequencies of GSTM1 null and GSTT1 null genotypes were 82/162 (50.6%) and 33/162 (20.4%), respectively. No any significant differences were observed between different genotype groups and clinicopathological parameters including Gleason score,
pathological stage, tumor volume, surgical margin status, extraprostatic extension, seminal vesicle invasion, perineural invasion or patient age. Although the roles of GSTM1 and GSTT1 deletions on prostate cancer risk was indicated in Turkish population, our results revealed that null or wild type genotypes of GSTM1 and GSTT1 genes are not associated with prognostic parameters in prostate cancer.